IMP-10482

IL1B

Recombinant Equine IL-1 beta/IL-1F2 protein (Ala116-Ala268, Glu179Gly, Met188Thr, Thr194Ile, Ser245Lys and Arg256Gln) without tag was expressed in E. coli.

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

E. coli

Equine

Tag Free

Ala116

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Predicted Molecular Mass: 17 kDa

Ala116-Ala268, Glu179Gly, Met188Thr, Thr194Ile, Ser245Lys and Arg256Gln

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 5-30 pg/mL.

<1.0 EU/μg of the protein by the LAL method.

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.