Recombinant Guinea Pig IL1B Protein (Thr115-Ser266)
Product Description
Cat
IMP-10486
Official Symbol
Il1b
Product Overview
Recombinant Guinea Pig IL-1 beta/IL-1F2 protein Thr115-Ser266, with an N-terminal Met was expressed in E. coli.
Description
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Expression System
E. coli
Species
Guinea Pig
Tag
Tag Free
Predicted N Terminal
Met
Form
Lyophilized from a 0.2 μm filtered solution in MES, NaCl, DTT, and EDTA with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 17.4 kDa
SDS-PAGE: 18 kDa, reducing conditions
Protein length
Thr115-Ser266
Bio-activity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 7-35 pg/mL.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Monomer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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