Recombinant Horse IL1b protein, N-His Tag

Product Description

Cat

IMP-2865

Official Symbol

IL1b

Product Overview

Recombinant Horse IL1b protein(His63~Val247), fused with N-terminal His Tag, was expressed in E. coli.

Expression System

E. coli

Species

Horse

Tag

N-His Tag

Form

PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Molecular Mass

25kDa

Protein length

His63~Val247

Purity

> 90%

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

IL-8 levels in the cell supernatant of A549 induced by IL-1β

Interleukin 1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1β can induced IL-8 production in A549 cells. To test the bioactivity of IL-1β, A549 cells were seeded into 24-well plate at a density of 1x105 cells/mL , and allowed to attach overnight before treated with certain concentrations of IL-1β for 24h and IL-8 levels in the cell supernatant were determined by ELISA

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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