Recombinant Human ACE2 protein, C-hFc tag

Product Description

Cat

IMP-846

Official Symbol

ACE2

Product Overview

Recombinant Human ACE2(Q9BYF1)(Gln18-Ser740) protein, fused with a Fc tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Human

Tag

C-hFc tag

Form

Liquid in sterile 20mM Tris–HCl, 300mM NaCl, 10% Glycerol, 100mM Glycine, pH 7.4.

Molecular Mass

The protein has a calculated MW of 110.5kDa, and migrates as 110-140kDa under reducing conditions.

Protein length

Gln18-Ser740

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Concentration

Please refer to the COA for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human ACE-2-Fc at 5μg/ml (100 μl/well)can bind 2019-nCoV S Protein RBD-His. The ED50 of 2019-nCoV S Protein RBD-His is 0.120 ug/ml.

Bioactivity-BLI 1

Loaded Human ACE-2-Fc on Protein A Biosensor, can bind 2019-nCoV S-trimer Protein-His with an affinity constant of 0.125 nM as determined in BLI assay.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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