Recombinant Human ACE2 protein, C-hFc tag

Product Description

Cat

IMP-846

Official Symbol

ACE2

Product Overview

Recombinant Human ACE2(Q9BYF1)(Gln18-Ser740) protein, fused with a Fc tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Human

Tag

C-hFc tag

Form

Liquid in sterile 20mM Tris–HCl, 300mM NaCl, 10% Glycerol, 100mM Glycine, pH 7.4.

Molecular Mass

The protein has a calculated MW of 110.5kDa, and migrates as 110-140kDa under reducing conditions.

Protein length

Gln18-Ser740

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Concentration

Please refer to the COA for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human ACE-2-Fc at 5μg/ml (100 μl/well)can bind 2019-nCoV S Protein RBD-His. The ED50 of 2019-nCoV S Protein RBD-His is 0.120 ug/ml.

Bioactivity-BLI 1

Loaded Human ACE-2-Fc on Protein A Biosensor, can bind 2019-nCoV S-trimer Protein-His with an affinity constant of 0.125 nM as determined in BLI assay.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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