IMP-9169

ACVR1

Human ACVR1 recombinant protein with polyhistidine tag at the C-terminus expressed in E. coli.

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. This gene encodes activin A type I receptor which signals a particular transcriptional response in concert with activin type II receptors. Mutations in this gene are associated with fibrodysplasia ossificans progressive.

E. coli

Human

C-His

Lyophilized from 0.1% sarkosyl, PBS, pH 8.0.

MGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS

< 0.1 EU/μg of the protein by the LAL method.

> 95% by SDS-PAGE

Functional Study; SDS-PAGE

Store at -20 centigrade, lyophilized protein is stable for 1 year. After reconstitution with deionized water, store at -20 to -80 centigrade. Aliquot to avoid repeated freezing and thawing.

SDS-PAGE Stained with Coomassie Blue

Measure by its ability to induce hemoglobin expression in K562 cells. The ED50 for this effect is <0.85 ng/mL.