Recombinant Human ACVR1B Protein (Leu32-Glu126), C-Fc and 6×His-tagged

Product Description

Cat

IMP-10187

Official Symbol

ACVR1B

Product Overview

Recombinant human Activin RIB/ALK-4 protein (Leu32-Glu126) with human IgG1 (Pro100-Lys330) Fc tag and 6×His tag was expressed in Mouse myeloma cell line

Description

This gene encodes an activin A type IB receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I and two type II receptors. This protein is a type I receptor which is essential for signaling. Mutations in this gene are associated with pituitary tumors. Alternate splicing results in multiple transcript variants.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-Fc and 6×His

Predicted N Terminal

Leu32

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 37.5 kDa (monomer)
SDS-PAGE: 45-50 kDa, reducing conditions

Protein length

Leu32-Glu126

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized rhActivin RIB/Fc Chimera at 2 μg/mL (100 μL/well) can bind rhCripto with a linear range of 0.8-100 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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