Recombinant Human ACVRL1 Protein, C-His-tagged

Product Description

Cat

IMP-1797

Official Symbol

ACVRL1

Product Overview

Recombinant the extracellular domain of human ALK1 (NP_000011.2) (Met 1-Gln 118) was fused with a polyhistide tag at the C-terminus.

Description

Activin A receptor, type II-like 1 (ACVRL1), also known as ALK-1 (activin receptor-like kinase 1), is an endothelial-specific type I receptor of the TGF-beta (transforming growth factor beta) receptor family of ligands. On ligand binding, a heteromeric receptor complex forms consisting of two type II and two type I transmembrane serine/threonine kinases. ACVRL1 protein is expressed in certain blood vessels of kidney, spleen, heart and intestine, serving as an important role during vascular development. Mutations in ACVRL1 gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 and vascular disease.

Expression System

HEK293

Species

Human

Tag

C-His

Predicted N Terminal

Asp 22

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant human ALK1 comprises 108 amino acids and has a predicted molecular mass of 12.3 kDa. As a result of glycosylation, rhALK1 migrates as an approximately 27 kDa protein in SDS-PAGE under reducing conditions.

Protein length

Met1-Gln118

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 92 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-Cell based assay 1

Measured by its ability to inhibit BMP9 induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. The ED50 for this effect is typically 50-200 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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