Recombinant Human AFM Protein (Leu22-Asn599), C-6×His-tagged

Product Description

Cat

IMP-10189

Official Symbol

AFM

Product Overview

Recombinant human Afamin protein (Leu22-Asn599) with a C-terminal 6×His tag was expressed in human embryonic kidney cell.

Description

This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.

Expression System

HEK293

Species

Human

Tag

C-6×His

Predicted N Terminal

Leu22

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 67 kDa
SDS-PAGE: 71-80 kDa

Protein length

Leu22-Asn599

Bio-activity

Measured by its ability to bind Biotinylated Recombinant Mouse Wnt-3a in a functional ELISA. The ED50 for this effect is 0.8-6.4 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry