Recombinant Human AGER Protein (24-342aa), C-hIgG-His-Tagged

Product Description

Cat

IMP-9514

Official Symbol

AGER

Product Overview

Recombinant human RAGE, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Description

RAGE, also known as advanced glycosylation end product-specific receptor isoform 1, is a member of the immunoglobulin super-family transmembrane proteins. It mediates interactions of advanced glycosylation end products (AGE). AGE/RAGE signaling plays an important role in regulating the production/expression of TNFalpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. This protein contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons.

Expression System

Baculovirus

Species

Human

Tag

C-hIgG-His

Form

Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Molecular Mass

61.2 kDa (561 aa)

Protein length

24-342

AA Sequence

<ADL>QNITARIGEPLVLKCKGAPKKPPQRLEWKLNTGRTEAWKVLSPQGGGPWDSVARVLPNGSLFLPAVGIQDEGIFRCQAMNRNGKETKSNYRVRVYQIPGKPEIVDSASELTAGVPNKVGTCVSEGSYPAGTLSWHLDGKPLVPNEKGVSVKEQTRRHPETGLFTLQSELMVTPARGGDPRPTFSCSFSPGLPRHRALRTAPIQPRVWEPVPLEEVQLVVEPEGGAVAPGGTVTLTCEVPAQPSPQIHWMKDGVPLPLPPSPVLILPEIGPQDQGTYSCVATHSSHGPQESRAVSISIIEPGEEGPTAGSVGGSGLGTLA<LEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLM><ISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQ><PREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEAL><HNHYTQKSLSLSPGKHHHHHH>

Endotoxin

< 1 EU/μg of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Applications

SDS-PAGE,Bioactivity

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.25 mg/mL (determined by BCA assay)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1

Human HMGB1 is coated at 20 μg/mL (100 μL/well) can bind Human RAGE. The ED50 range ≤15 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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