Recombinant Human AKT1 Protein (104-480), His-tagged
Product Description
Cat
IMP-8129
Official Symbol
AKT1
Product Overview
Human AKT1 (NP_005154.1, 104 a.a.-480 a.a.) partial recombinant protein with His tag was expressed in baculovirus infected Sf21 cells.
Description
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Expression System
Insect cells
Species
Human
Tag
His
Form
Liquid, In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Molecular Mass
70 kDa
Protein length
104-480
Purity
69 % by SDS-PAGE/CBB staining
Applications
Functional Study; SDS-PAGE
Storage
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
SDS-PAGE
Quality Control Testing

Loading 1 μg protein in SDS-PAGE

Bioactivity-ELISA 1
Result of activity analysis

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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