Recombinant Human AKT1 Protein (106-480), GST/His-tagged
Product Description
Cat
IMP-8127
Official Symbol
AKT1
Product Overview
Human AKT1 (NM_005163, 106 a.a.-480 a.a) partial recombinant protein with GST-His tag expressed in Sf9 cells.
Description
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Expression System
Insect cells
Species
Human
Tag
GST/His
Form
Liquid, In 50 mM Tris-HCl, 100 mM NaCl, pH 8.0. (5 mM DTT, 15 mM reduced glutathione, 20% glycerol)
Molecular Mass
77.832 kDa
Protein length
106-480
Applications
Functional Study; SDS-PAGE
Storage
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Concentration
0.434 μg/μL
SDS-PAGE
Quality Control Testing

2 μg/lane SDS-PAGE Stained with Coomassie Blue

Bioactivity-ELISA 1
Result of activity analysis

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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