Recombinant Human AKT1 Protein (64-420), His-tagged
Product Description
Cat
IMP-8128
Official Symbol
AKT1
Product Overview
Human AKT1 (NP_004842, 64 a.a.-420 a.a ) partial recombinant protein with His tag was expressed in E. coli.
Description
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Expression System
E. coli
Species
Human
Tag
His
Form
Liquid, In 20mM Tris-HCl buffer, pH8.0 (10% glycerol).
Molecular Mass
40.9 kDa
Protein length
64-420
AA Sequence
MGSSHHHHHHSSGLVPRGSHMGSKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG
Purity
> 90% by SDS-PAGE
Applications
SDS-PAGE
Storage
Store at 2 centigrade to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Aliquot to avoid repeated freezing and thawing.
SDS-PAGE
Quality Control Testing

SDS-PAGE analysis of AKT1 (Human) Recombinant Protein, 3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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