Recombinant Human AKT1 Protein, GST/His-tagged

Product Description

Cat

IMP-6452

Official Symbol

AKT1

Product Overview

Recombinant AKT1 (accession number NP_005154.2) activated with PDK1, was expressed in Sf9 cells as an N-terminal GST-HIS6 fusion protein with a 3C cleavage site.

Description

AKT1 is a serine-threonine protein kinase that is activated by Platelet-Derived Growth Factor (PDGF) and PI 3-kinase signaling pathways. AKT has been shown to be a critical mediator of survival and apoptosis signaling events. Activation of AKT1 involves (and requires) phosphorylation of serine 473. AKT is also referred to as Protein Kinase B (PKB) and RAC.

Expression System

Insect cells

Species

Human

Tag

GST/His

Form

Full length recombinant AKT1 protein expressed in Sf9 cells and supplied in 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.

Molecular Mass

~ 81 kDa

Applications

Kinase assays

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

SDS-PAGE

AKT1 protein was run on an SDS-PAGE gel and stained with Coomassie blue.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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