IMP-1813

ANGPT2

Recombinant human ANGPT2 (NP_001138.1) (Met1-Phe496) was expressed with a polyhistidine tag at the C-terminus.

Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.

CHO

Human

C-His

Tyr 19

Lyophilized from sterile 20 mM MOPS, 150 mM NaCl, 0.05 % CHAPS, pH 7.0, 5 % trehalose, 5% mannitol and 0.01% Tween80.

The recombinant human ANGPT2 consists of 489 amino acids and predicts a molecular mass of 56.4 kDa. As a result of glycosylation, it migrates as an approximately 62.6 kDa band in SDS-PAGE under reducing conditions.

Met1-Phe496

< 1.0 EU/μg of the protein as determined by the LAL method

> 95 % as determined by SDS-PAGE

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Immobilized Human Ang2 His at 2 μg/mL (100 μL/well) can bind Human TIE2 Fch, the EC50 of Human TIE2 Fch is 80-480 ng/mL.