Recombinant Human ANGPT2 Protein (Tyr19-Phe496), C-6×His-tagged, Biotinylated
Product Description
Cat
IMP-10592
Official Symbol
ANGPT2
Product Overview
Biotinylated recombinant human Angiopoietin-2 protein (Tyr19-Phe496) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
This gene belongs to the angiopoietin family of growth factors. The protein encoded by this gene is an antagonist of angiopoietin 1, and both angiopoietin 1 and angiopoietin 2 are ligands for the endothelial TEK receptor tyrosine kinase. Angiopoietin 2 is upregulated in multiple inflammatory diseases and is implicated in the direct control of inflammation-related signaling pathways. The encoded protein affects angiogenesis during embryogenesis and tumorigenesis, disrupts the vascular remodeling ability of angiopoietin 1, and may induce endothelial cell apoptosis. This gene serves a prognostic biomarker for acute respiratory distress syndrome.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-6×His
Predicted N Terminal
Tyr19
Form
Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, CHAPS and PEG with BSA as a carrier protein.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 56 kDa (unlabeled)
Protein length
Tyr19-Phe496
Bio-activity
Measured by its ability to activate Tie-2 in C6 rat glial cells transfected with human Tie-2. 0.2 μg/mL of Biotinylated Recombinant Human Angiopoietin-2 significantly induces phosphorylation of human Tie-2.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Oligomer, biotinylated via sugar residues
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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