Recombinant Human ANGPTL4 Protein (Leu165-Ser406), C-6×His-tagged
Product Description
Cat
IMP-10195
Official Symbol
ANGPTL4
Product Overview
Recombinant human Angiopoietin-like 4 C-Terminal Frag (Leu165-Ser406) , with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-6×His
Predicted N Terminal
Leu165
Form
Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Molecular Mass
Predicted Molecular Mass: 28 kDa
SDS-PAGE: 36 kDa, reducing conditions
Protein length
Leu165-Ser406
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Angiopoietin-like 4/ANGPTL4 C-Terminal Fragment is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera binds with an ED50 of 70.0-350 ng/mL.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Oligomer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 250 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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