Recombinant Human ANGPTL7 Protein (Gln27-Pro346), C-10×His-tagged, Biotinylated
Product Description
Cat
IMP-10594
Official Symbol
ANGPTL7
Product Overview
Biotinylated recombinant human Angiopoietin-like 7 protein (Gln27-Pro346) with a C-terminal 10-His tag was expressed in Chinese Hamster Ovary cell line.
Description
Enables identical protein binding activity. Involved in negative regulation of vasculature development involved in avascular cornea development in camera-type eye and regulation of extracellular matrix organization. Located in extracellular region.
Expression System
CHO
Species
Human
Tag
C-10×His
Predicted N Terminal
No results: Gln27 predicted, sequencing might be blocked
Form
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 39 kDa (unlabeled)
SDS-PAGE: >110 kDa, reducing conditions
Protein length
Gln27-Pro346
Bio-activity
Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand, Recombinant Mouse Thrombopoietin/Tpo, and Recombinant Mouse Flt-3 Ligand. The ED50 for this effect is 50-200 ng/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE with silver staining.
Notes
Oligomer, Biotinylated on protein carbohydrates
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand, Recombinant Mouse Thrombopoietin/Tpo, and Recombinant Mouse Flt-3 Ligand. The ED50 for this effect is 50-200 ng/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info