Recombinant Human ANGPTL7 Protein (Gln27-Pro346), C-10×His-tagged, Biotinylated

Product Description

Cat

IMP-10594

Official Symbol

ANGPTL7

Product Overview

Biotinylated recombinant human Angiopoietin-like 7 protein (Gln27-Pro346) with a C-terminal 10-His tag was expressed in Chinese Hamster Ovary cell line.

Description

Enables identical protein binding activity. Involved in negative regulation of vasculature development involved in avascular cornea development in camera-type eye and regulation of extracellular matrix organization. Located in extracellular region.

Expression System

CHO

Species

Human

Tag

C-10×His

Predicted N Terminal

No results: Gln27 predicted, sequencing might be blocked

Form

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 39 kDa (unlabeled)
SDS-PAGE: >110 kDa, reducing conditions

Protein length

Gln27-Pro346

Bio-activity

Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand, Recombinant Mouse Thrombopoietin/Tpo, and Recombinant Mouse Flt-3 Ligand. The ED50 for this effect is 50-200 ng/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE with silver staining.

Notes

Oligomer, Biotinylated on protein carbohydrates

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in PBS.

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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