Recombinant Human ANGPTL7 Protein (Met1-Pro346), C-10×His-tagged

Product Description

Cat

IMP-10200

Official Symbol

ANGPTL7

Product Overview

Recombinant human Angiopoietin-like 7 protein (Met1-Pro346) with a C-terminal 10-His tag was expressed in Chinese Hamster Ovary cell line.

Description

Enables identical protein binding activity. Involved in negative regulation of vasculature development involved in avascular cornea development in camera-type eye and regulation of extracellular matrix organization. Located in extracellular region.

Expression System

CHO

Species

Human

Tag

C-10×His

Predicted N Terminal

No results obtained: Gln27 predicted

Form

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 38.6 kDa
SDS-PAGE: 49-55 kDa, reducing conditions

Protein length

Met1-Pro346

Bio-activity

Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand, Recombinant Mouse Thrombopoietin/Tpo, and Recombinant Mouse Flt-3 Ligand. The ED50 for this effect is 50-200 ng/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

Online Inquiry