Recombinant Human ANGPTL7 Protein (Met1-Pro346), C-10×His-tagged

Product Description

Cat

IMP-10200

Official Symbol

ANGPTL7

Product Overview

Recombinant human Angiopoietin-like 7 protein (Met1-Pro346) with a C-terminal 10-His tag was expressed in Chinese Hamster Ovary cell line.

Description

Enables identical protein binding activity. Involved in negative regulation of vasculature development involved in avascular cornea development in camera-type eye and regulation of extracellular matrix organization. Located in extracellular region.

Expression System

CHO

Species

Human

Tag

C-10×His

Predicted N Terminal

No results obtained: Gln27 predicted

Form

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 38.6 kDa
SDS-PAGE: 49-55 kDa, reducing conditions

Protein length

Met1-Pro346

Bio-activity

Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand, Recombinant Mouse Thrombopoietin/Tpo, and Recombinant Mouse Flt-3 Ligand. The ED50 for this effect is 50-200 ng/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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