Recombinant Human AXL protein(26-449aa), fused with C-terminal mFc Tag, was expressed in HEK293.
Expression System
HEK293
Species
Human
Tag
C-mFc Tag
Form
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
Molecular Mass
The protein has a predicted molecular mass of 72.0 kDa after removal of the signal peptide.The apparent molecular mass of AXL-mFc is approximately 100-130 kDa due to glycosylation.
Protein length
26-449aa
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Biological Activity
ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human AXL, mFc tagged protein can bind Anti-AXL Neutralizing Antibody in a linear range of 3.2-80.0 ng/ml.
Storage
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized proteins are shipped at ambient temperature.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
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