Recombinant Human B3GAT1 Protein (His25-Ile334), N-6×His-tagged
Product Description
Cat
IMP-10635
Official Symbol
B3GAT1
Product Overview
Recombinant human B3GAT1 protein His25-Ile334, with N-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized.
Expression System
CHO
Species
Human
Tag
N-6×His
Predicted N Terminal
His
Form
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Molecular Mass
Predicted Molecular Mass: 36 kDa
SDS-PAGE: 50-59 kDa, reducing conditions
Protein length
His25-Ile334
Bio-activity
Measured by its ability to transfer GlcA from UDP-GlcA to lactose. The specific activity is >750 pmol/min/μg, as measured under the described conditions.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain at 5 μg per lane
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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