Recombinant Human B3GAT1 Protein (His25-Ile334), N-6×His-tagged
Product Description
Cat
IMP-10635
Official Symbol
B3GAT1
Product Overview
Recombinant human B3GAT1 protein His25-Ile334, with N-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized.
Expression System
CHO
Species
Human
Tag
N-6×His
Predicted N Terminal
His
Form
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Molecular Mass
Predicted Molecular Mass: 36 kDa
SDS-PAGE: 50-59 kDa, reducing conditions
Protein length
His25-Ile334
Bio-activity
Measured by its ability to transfer GlcA from UDP-GlcA to lactose. The specific activity is >750 pmol/min/μg, as measured under the described conditions.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain at 5 μg per lane
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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