IMP-1595

BIN1

Recombinant human BIN1 isoform BIN1-13 (O00499-10) (Met 1-Pro 424) was expressed, with a polyhistidine tag at the N-terminus.

Amphiphysin 2, also named bridging integrator-1 (BIN1) or SH3P9, Amphiphysin2/BIN1 is a crescent-shaped N-BAR protein playing a key role in forming deeply invaginated tubes in muscle T-tubules, has been recently implicated in rare and common diseases affecting different tissues and physiological functions. BIN1 downregulation is linked to cancer progression and also correlates with ventricular cardiomyopathy and arrhythmia preceding heart failure. Increased BIN1 expression is linked to increased susceptibility for late-onset Alzheimer's disease. In addition, altered splicing may account for the muscle component of myotonic dystrophies, while recessive germinal mutations cause centronuclear myopathy. Bridging integrator 1 (BIN1) as a late-onset Alzheimer's disease (AD) susceptibility gene. There are at least 15 different known isoforms of BIN1, with many being expressed in the brain including the longest isoform (iso1), which is brain-specific and localizes to axon initial segments and nodes of Ranvier. The bridging integrator 1 (BIN1) tumor suppressor encodes multiple alternatively spliced isoforms implicated in DNA repair, cell-cycle control, apoptosis and membrane dynamics.

E. coli

Human

N-His

Met

Lyophilized from sterile 20mM Tris, 10% glycerol, 1mM DDT, pH 8.0, 5 % trehalose, 5% mannitol and 0.01% Tween80.

The recombinant human BIN1 comprises 439 amino acids and has a predicted molecular mass of 49.3 kDa. It migrates as an approximately 52 kDa band in SDS-PAGE under reducing conditions.

Met1-Pro424

Please contact us for more information.

> 85 % as determined by SDS-PAGE

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.