Recombinant Human BTLA protein, C-Fc Tag
Product Description
Cat
IMP-261
Official Symbol
BTLA
Product Overview
Recombinant Human BTLA protein(AA Lys 31-Thr 134)(Accession # NP_001078826.1) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.
Expression System
HEK293
Species
Human
Tag
C-Fc Tag
Form
Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 38.7 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Lys 31-Thr 134
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>92% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Human BTLA, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human HVEM, His Tag at 5 ug/mL (100 uL/well) can bind Human BTLA, Fc Tag with a linear range of 0.02-1.25 ug/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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