Recombinant Human BTLA Protein, C-hFc& AVI-tagged, Biotinylated

Product Description

Cat

IMP-1394

Official Symbol

BTLA

Product Overview

Recombinant human BTLA (NP_001078826.1) (Met1-Thr134) was expressed with a c-terminal Fc region of human IgG1 tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Description

BTLA is a inhibitory molecule which belongs to the Ig superfamily. It down-modulates immune responses. As such, reagents that regulate the binding of BTLA to its ligand or alter BTLA signaling have significant therapeutic promise. BTLA is crucial to understand the mechanism(s) of action of these antibodies before attempting clinical applications. BTLA is not expressed by naive T cells, but it is induced during activation and remains expressed on T helper type 1 (T(H)1) but not T(H)2 cells. BTLA is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1).

Expression System

HEK293

Species

Human

Tag

C-hFc& AVI

Predicted N Terminal

Lys 31

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant human BTLA consists of 357 amino acids and predicts a molecular mass of 40.6 kDa.

Protein length

Met1-Thr134

Endotoxin

< 1.0 EU/μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human HVEM His at 2 μg/mL (100 μL/well) can bind Human BTLA (hFc&AVI Tag), Biotinylated, the EC50 is 300-1800 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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