Recombinant Human BTLA protein, C-hFc-Myc Tag

Product Description

Cat

IMP-6022

Official Symbol

BTLA

Product Overview

Recombinant Human BTLA protein(Q7Z6A9)(31-150aa), fused with C-terminal hFc-Myc tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-hFc-Myc Tag

Form

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Molecular Mass

43.9 kDa

Protein length

31-150aa

AA Sequence

KESCDVQLYIKRQSEHSILAGDPFELECPVKYCANRPHVTWCKLNGTTCVKLEDRQTSWKEEKNISFFILHFEPVLPNDNGSYRCSANFQSNLIESHSTTLYVTDVKSASERPSKDEMAS

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Purity

Greater than 94% as determined by SDS-PAGE.

Biological Activity

①Measured by its binding ability in a functional ELISA. Immobilized BTLA at 5 μg/ml can bind biotinylated human TNFRSF14, the EC50 is 137.8-233.4 ng/ml.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized BTLA at 5 ug/ml can bind biotinylated human TNFRSF14, the EC50 is 137.8-233.4 ng/ml

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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