Recombinant Human BTLA Protein, C-hFc-tagged
Product Description
Cat
IMP-1388
Official Symbol
BTLA
Product Overview
Recombinant human BTLA (NP_001078826.1) (Met1-Thr134) was expressed with the Fc region of human IgG1 at the C-terminus.
Description
BTLA is a inhibitory molecule which belongs to the Ig superfamily. It down-modulates immune responses. As such, reagents that regulate the binding of BTLA to its ligand or alter BTLA signaling have significant therapeutic promise. BTLA is crucial to understand the mechanism(s) of action of these antibodies before attempting clinical applications. BTLA is not expressed by naive T cells, but it is induced during activation and remains expressed on T helper type 1 (T(H)1) but not T(H)2 cells. BTLA is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1).
Expression System
HEK293
Species
Human
Tag
C-hFc
Predicted N Terminal
Lys 31
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human BTLA comprises 342 amino acids and has a predicted molecular mass 38.8 kDa. As a result of glycosylation, it migrates as an approximately 53.7 kDa band in SDS-PAGE under reducing conditions.
Protein length
Met1-Thr134
Endotoxin
< 1.0 EU/μg protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Bioactivity-ELISA 1
Bioactivity-ELISA

Measured by its binding ability in a functional ELISA. Immobilized human BTLA-Fc at 10 μg/mL (100 μL/well) can bind biotinylated mouse HVEM-Fch, the EC50 of biotinylated mouse HVEM-Fch is 6-70 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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