Recombinant Human BTLA Protein (Ile25-Ser150), C-Fc-tagged
Product Description
Cat
IMP-10165
Official Symbol
BTLA
Product Overview
Recombinant human BTLA protein (Ile25-Ser150) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-Fc
Predicted N Terminal
Ile25
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 41 kDa
SDS-PAGE: 53-65 kDa, reducing conditions
Protein length
Ile25-Ser150
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human BTLA Fc Chimera that produces 50% of the optimal binding response is approximately 0.25-1.25 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>85%, by SDS-PAGE with silver staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human BTLA Fc Chimera that produces 50% of the optimal binding response is approximately 0.25-1.25 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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