Recombinant Human BTN1A1 Protein (Ala27-Arg242), C-6×His and Avi-tagged, Biotinylated conjugated
Product Description
Cat
IMP-10169
Official Symbol
BTN1A1
Product Overview
Biotinylated recombinant human BTN1A1/Butyrophilin protein (Ala27-Arg242) with 6×His tag and Avi-tag was expressed in human embryonic kidney cell.
Description
Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families.
Expression System
HEK293
Species
Human
Tag
C-6×His and Avi
Predicted N Terminal
Ala27
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 27 kDa
SDS-PAGE: 30-43 kDa, under reducing conditions
Protein length
Ala27-Arg242
Bio-activity
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL.
Measured by its binding ability in a functional ELISA. When Rabbit Anti-Human BTN1A1/Butyrophilin Monoclonal Antibody is immobilized at 10 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human BTN1A1/Butyrophilin His-tag Avi-tag that produces 50% of the optimal binding response is 0.01-0.1 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Biotinylated protein via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL. Measured by its binding ability in a functional ELISA. When Rabbit Anti-Human BTN1A1/Butyrophilin Monoclonal Antibody is immobilized at 10 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human BTN1A1/Butyrophilin His-tag Avi-tag that produces 50% of the optimal binding response is 0.01-0.1 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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