Recombinant Human BTN1A1 Protein (Ala27-Arg242), C-6×His and Avi-tagged, Biotinylated conjugated

Product Description

Cat

IMP-10169

Official Symbol

BTN1A1

Product Overview

Biotinylated recombinant human BTN1A1/Butyrophilin protein (Ala27-Arg242) with 6×His tag and Avi-tag was expressed in human embryonic kidney cell.

Description

Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families.

Expression System

HEK293

Species

Human

Tag

C-6×His and Avi

Predicted N Terminal

Ala27

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 27 kDa
SDS-PAGE: 30-43 kDa, under reducing conditions

Protein length

Ala27-Arg242

Bio-activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL.
Measured by its binding ability in a functional ELISA. When Rabbit Anti-Human BTN1A1/Butyrophilin Monoclonal Antibody is immobilized at 10 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human BTN1A1/Butyrophilin His-tag Avi-tag that produces 50% of the optimal binding response is 0.01-0.1 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Biotinylated protein via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL. Measured by its binding ability in a functional ELISA. When Rabbit Anti-Human BTN1A1/Butyrophilin Monoclonal Antibody is immobilized at 10 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human BTN1A1/Butyrophilin His-tag Avi-tag that produces 50% of the optimal binding response is 0.01-0.1 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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