Recombinant Human BTN1A1 Protein (Ala27-Arg242), C-6×His-tagged
Product Description
Cat
IMP-10168
Official Symbol
BTN1A1
Product Overview
Recombinant human BTN1A1/Butyrophilin protein (Ala27-Arg242) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-6×His
Predicted N Terminal
Ala27
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 25 kDa
SDS-PAGE: 33-41 kDa, reducing conditions
Protein length
Ala27-Arg242
Bio-activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-2.5 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE with silver staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-2.5 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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