Recombinant Human BTN3A2 Protein (Gln30-Trp248), C-6×His and Avi-tagged, Biotinylated conjugated
Product Description
Cat
IMP-10177
Official Symbol
BTN3A2
Product Overview
Biotinylated recombinant human BTN3A2 protein (Gln30-Trp248) with 6×His-tag and Avi-tag at C-terminus was expresssed in human embryonic kidney cell.
Description
This gene encodes a member of the immunoglobulin superfamily, which resides in the juxta-telomeric region of the major histocompatability class 1 locus and is clustered with the other family members on chromosome 6. The encoded protein may be involved in the adaptive immune response. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Expression System
HEK293
Species
Human
Tag
C-6×His and Avi
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 26 kDa
SDS-PAGE: 27-37 kDa, under reducing conditions
Protein length
Gln30-Trp248
Bio-activity
Measured by its binding ability in a functional ELISA. When Sheep Anti-Human BTN3A1/2 Antigen Affinity-purified Polyclonal Antibody is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human BTN3A2 His-tag Avi-tag that produces 50% of the optimal binding response is approximately 15-75 ng/mL. Measured by its ability to enhance anti-CD3-induced IFN-gamma secretion of mouse CD3+ T cells. The ED50 for this effect is 1.5-9.0 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Sheep Anti-Human BTN3A1/2 Antigen Affinity-purified Polyclonal Antibody is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human BTN3A2 His-tag Avi-tag that produces 50% of the optimal binding response is approximately 15-75 ng/mL. Measured by its ability to enhance anti-CD3-induced IFN-gamma secretion of mouse CD3+ T cells. The ED50 for this effect is 1.5-9.0 μg/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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