Recombinant Human BTN3A3 Protein, C-mFc-tagged

Product Description

Cat

IMP-1528

Official Symbol

BTN3A3

Product Overview

Recombinant human BTN3A3 (O00478-1) (Met1-Trp248) was fused with Fc region of mouse IgG at the C-terminus.

Description

The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. The butyrophilin 3 (BTN3) receptors are implicated in the T lymphocytes regulation and present a wide plasticity in mammals. A thorough phylogenetic analysis reveals a concerted evolution of BTN3 characterized by a strong and recurrent homogenization of the region encoding the signal peptide and the immunoglobulin variable (IgV) domain in Hominoids, where the sequences of BTN3A1 or BTN3A3 are replaced by BTN3A2 sequence.

Expression System

HEK293

Species

Human

Tag

C-mFc

Predicted N Terminal

Gln 30

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant human BTN3A3/mFc is a disulfide-linked homodimer. The reduced monomer comprises 453 amino acids and has a predicted molecular mass of 50 kDa. The apparent molecular mass of the protein is approximately 53 in SDS-PAGE under reducing conditions.

Protein length

Met1-Trp248

Endotoxin

< 1.0 EU/μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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