Recombinant Human C5AR1 Protein, C-Myc/DDK-tagged

Product Description

Cat

IMP-6781

Official Symbol

C5ar1

Product Overview

Recombinant protein of human complement component 5a receptor 1 (C5AR1) with a C-Myc/DDK tag was expressed in HEK293T.

Description

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a (PubMed: 1847994, PubMed: 8182049, PubMed: 7622471, PubMed: 9553099, PubMed: 10636859, PubMed: 15153520, PubMed: 29300009). The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events (PubMed: 8182049, PubMed: 7622471, PubMed: 9553099). Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production (PubMed: 10636859, PubMed: 15153520).[UniProtKB/Swiss-Prot Function]

Expression System

HEK293T

Species

Human

Tag

C-Myc/DDK

Form

25mM Tris.HCl, pH 7.3, 100mM glycine, 10% glycerol

Molecular Mass

39.1 kDa

AA Sequence

MNSFNYTTPDYGHYDDKDTLDLNTPVDKTSNTLRVPDILALVIFAVVFLVGVLGNALVVWVTAFEAKRTINAIWFLNLAVADFLSCLALPILFTSIVQHHHWPFGGAACSILPSLILLNMYASILLLATISADRFLLVFKPIWCQNFRGAGLAWIACAVAWGLALLLTIPSFLYRVVREEYFPPKVLCGVDYSHDKRRERAVAIVRLVLGFLWPLLTLTICYTFILLRTWSRRATRSTKTLKVVVAVVASFFIFWLPYQVTGIMMSFLEPSSPTFLLLNKLDSLCVSFAYINCCINPIIYVVAGQGFQGRLRKSLPSLLRNVLTEESVVRESKSFTRSTVDTMAQKTQAVTRTRPLEQKLISEEDLAANDILDYKDDDDKV

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining.

Storage

Store at -80 centigrade after receiving vials.
Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Concentration

> 50 μg/mL as determined by microplate BCA method.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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