Recombinant Human CARTPT Protein, C-mFc-tagged
Product Description
Cat
IMP-1878
Official Symbol
CARTPT
Product Overview
Recombinant human CARTPT (NP_004282.1) (Met1-Leu116) was expressed with the Fc region of mouse IgG1 at the C-terminus.
Description
In RNAseq data from human islet donors,  CARTPT expression levels correlated with insulin, exocytosis genes and key beta-cell transcription factors.  Insufficient insulin release and hyperglucagonaemia are culprits in type 2 diabetes. Cocaine-and amphetamine-regulated transcript (CART, encoded by Cartpt) affects islet hormone secretion and beta cell survival in vitro in rats, and Cart (-/-) mice have diminished insulin secretion. Established a potential role for cocaine and amphetamine regulated transcript (CARTPT) in dominant follicle selection in cattle. CARTPT expression is elevated in subordinate versus dominant follicles, and treatment with the mature form of the CARTPT peptide (CART) decreases follicle-stimulating hormone (FSH)-stimulated granulosa cell estradiol production in vitro and follicular fluid estradiol and granulosa cell CYP19A1 mRNA in vivo.
Expression System
HEK293
Species
Human
Tag
C-mFc
Predicted N Terminal
Gln 28
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human CARTPT/mFc comprises 323 amino acids and has a predicted molecular mass of 36.3 kDa. The apparent molecular mass of the monomer is approximately 37 kDa in SDS-PAGE under reducing conditions.
Protein length
Met1-Leu116
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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