Recombinant Human CCN2 Protein (Gln27-Ala349)
Product Description
Cat
IMP-10227
Official Symbol
CCN2
Product Overview
Recombinant human CTGF/CCN2 protein (Gln27-Ala349) without tag was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.
Expression System
Mouse myeloma cell line
Species
Human
Tag
Tag Free
Predicted N Terminal
No results obtained. Gln27 inferred from enzymatic pyroglutamate treatment revealing Asn28
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 35 kDa
SDS-PAGE: 35-45 kDa, reducing conditions
Protein length
Gln27-Ala349
Bio-activity
Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED50 for this effect is1.5-7.5 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED50 for this effect is1.5-7.5 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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