Recombinant Human CCN2 Protein (Gln27-Ala349)
Product Description
Cat
IMP-10227
Official Symbol
CCN2
Product Overview
Recombinant human CTGF/CCN2 protein (Gln27-Ala349) without tag was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.
Expression System
Mouse myeloma cell line
Species
Human
Tag
Tag Free
Predicted N Terminal
No results obtained. Gln27 inferred from enzymatic pyroglutamate treatment revealing Asn28
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 35 kDa
SDS-PAGE: 35-45 kDa, reducing conditions
Protein length
Gln27-Ala349
Bio-activity
Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED50 for this effect is1.5-7.5 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED50 for this effect is1.5-7.5 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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