Recombinant Human CD14 Protein (20-344aa), C-His-Tagged

Product Description

Cat

IMP-9538

Official Symbol

CD14

Product Overview

Recombinant human CD14, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Description

CD14, also known as monocyte differentiation antigen CD14, is a member of the eucine-rich repeat (LRR) proteins family. It exists in two forms, one anchored to the membrane by a GPI-tail (mCD14), the other a soluble form (sCD14). It is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide(LPS). It acts as a coreceptor (along with TLR4 and MD-2) and helps to detect bacteria in the body by binding LPS, a pathogenassociated molecular pattern (PAMP).

Expression System

HEK293

Species

Human

Tag

C-His

Form

Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Molecular Mass

35.8 kDa (331 aa)

Protein length

20-344

AA Sequence

TTPEPCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRVDADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKELTLEDLKITGTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQWLKPGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERGLMAALCPHKFPAIQNLALRNTGMETPTGVCAALAAAGVQPHSLDLSHNSLRATVNPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKLRVLDLSCNRLNRAPQPDELPEVDNLTLDGNPFLVPGTALPHEGSM<HHHHHH>

Endotoxin

< 1 EU/μg of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Applications

SDS-PAGE, Bioactivity

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

1 mg/mL (determined by Absorbance at 280nm)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1

Human CD14 enhance the IL-8 secretion of the THP‑1 human acute monocytic leukemia cells in the presence of LPS. The ED50 range ≤ 100 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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