Recombinant Human CD14 Protein (20-349aa), N-His-Tagged

Product Description

Cat

IMP-9352

Official Symbol

CD14

Product Overview

Recombinant human CD14 protein, fused to His-tag at Nterminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Description

CD14 is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.

Expression System

E. coli

Species

Human

Tag

N-His

Form

Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 30% glycerol 0.15M NaCl, 1mM DTT

Molecular Mass

37.9 kDa (353 aa) confirmed by MALDI-TOF

Protein length

20-349

AA Sequence

MGSSHHHHHHSSGLVPRGSHMGSTTPEPCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRVDADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKELTLEDLKITGTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQWLKPGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERGLMAALCPHKFPAIQNLALRNTGMETPTGVCAALAAAGVQPHSLDLSHNSLRATVNPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKLRVLDLSCNRLNRAPQPDELPEVDNLTLDGNPFLVPGTALPHEGSMNSGVV

Purity

> 90% by SDS-PAGE

Applications

SDS-PAGE

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.5 mg/mL (determined by Bradford assay)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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