IMP-1891

CD14

Recombinant extracellular domain (Thr 20-Cys 352) of the mature form of human CD14 (NP_000582.1) was fused to the Fc region of human IgG1 at the N-terminus.

The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is to serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns.

HEK293

Human

N-hFc

Glu 20

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

The recombinant human Fc/CD14 chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 570 amino acids and has a calculated molecular mass of 62.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhFc/CD14 monomer is approximately 75-85 kDa due to the glycosylation.

Thr20-Cys352

< 1.0 EU/μg of the protein as determined by the LAL method

> 97 % as determined by SDS-PAGE

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.