Recombinant Human CD2 Protein (Lys25-Asp209), C-hFc-tagged

Product Description

Cat

IMP-10253

Official Symbol

CD2

Product Overview

Recombinant human CD2 protein (Lys25-Asp209) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Human embryonic kidney cell

Description

The protein encoded by this gene is a surface antigen found on all peripheral blood T-cells. The encoded protein interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3' flanking sequence of this gene.

Expression System

HEK293

Species

Human

Tag

C-hFc

Predicted N Terminal

Lys25

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Molecular Mass

Predicted Molecular Mass: 48 kDa
SDS-PAGE: 59-65 kDa, reducing conditions

Protein length

Lys25-Asp209

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD58 Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human CD2 Fc Chimera binds with an ED50 of 1-5 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in PBS.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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