Recombinant Human CD200 Protein (Met1-Gly232), C-6×His-tagged
Product Description
Cat
IMP-10257
Official Symbol
CD200
Product Overview
Recombinant human CD200 protein (Met1-Gly232) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms.
Expression System
CHO
Species
Human
Tag
C-6×His
Predicted N Terminal
No results obtained. Gln31 inferred from enzymatic pyroglutamate treatment revealing Val32.
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 23 kDa
SDS-PAGE: 38-54 kDa, reducing conditions
Protein length
Met1-Gly232
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD200 R1 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), Recombinant Human CD200 binds with an ED50 of 0.2-1.2 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD200 R1 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), Recombinant Human CD200 binds with an ED50 of 0.2-1.2 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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