Recombinant Human CD200 Protein (Met1-Gly232), C-6×His-tagged
Product Description
Cat
IMP-10257
Official Symbol
CD200
Product Overview
Recombinant human CD200 protein (Met1-Gly232) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms.
Expression System
CHO
Species
Human
Tag
C-6×His
Predicted N Terminal
No results obtained. Gln31 inferred from enzymatic pyroglutamate treatment revealing Val32.
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 23 kDa
SDS-PAGE: 38-54 kDa, reducing conditions
Protein length
Met1-Gly232
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD200 R1 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), Recombinant Human CD200 binds with an ED50 of 0.2-1.2 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD200 R1 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), Recombinant Human CD200 binds with an ED50 of 0.2-1.2 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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