Recombinant Human CD22 Protein (Asp20-Arg687), C-6×His and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10269

Official Symbol

CD22

Product Overview

Biotinylated recombinant human Siglec-2/CD22 protein (Asp20-Arg687) with 6×His tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome.

Expression System

CHO

Species

Human

Tag

C-6×His and Avi

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 79 kDa
SDS-PAGE: 92-118 kDa, under reducing conditions

Protein length

Asp20-Arg687

Bio-activity

Measured by its binding ability in a functional ELISA. When Siglec-2/CD22 Antibody (hL22 (Epratuzumab))-Chimeric is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human Siglec-2/CD22 His-tag Avi-tag binds with an ED50 of 0.3-2.4 ng/mL. Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is 0.1-0.8 μg/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Biotinylated via Avi-tag.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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