Recombinant Human CD22 Protein (Asp20-Arg687), C-Fc-tagged, Alexa Fluor 488 Conjugated
Product Description
Cat
IMP-10109
Official Symbol
CD22
Product Overview
Alexa Fluor 488 conjugated recombinant human Siglec-2/CD22 protein (Asp20-Arg687) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Labeled with Alexa Fluor 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm
Description
Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-Fc
Predicted N Terminal
Asp20
Form
Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Conjugate
Alexa Fluor 488
Molecular Mass
Predicted Molecular Mass: 101.9 kDa (monomer)
SDS-PAGE: 128-141kDa, under reducing conditions.
Protein length
Asp20-Arg687
Bio-activity
Measured by flow cytometry for its ability to bind anti-human Siglec-2/CD22 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human Siglec-2/CD22 Fc Chimera Alexa Fluor 488 that produces 50% of the binding response is 50.0-500 ng/mL.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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