Recombinant Human CD22 Protein (Asp20-Arg687), C-Fc-tagged, Alexa Fluor 488 Conjugated

Product Description

Cat

IMP-10109

Official Symbol

CD22

Product Overview

Alexa Fluor 488 conjugated recombinant human Siglec-2/CD22 protein (Asp20-Arg687) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Labeled with Alexa Fluor 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm

Description

Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-Fc

Predicted N Terminal

Asp20

Form

Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Conjugate

Alexa Fluor 488

Molecular Mass

Predicted Molecular Mass: 101.9 kDa (monomer)
SDS-PAGE: 128-141kDa, under reducing conditions.

Protein length

Asp20-Arg687

Bio-activity

Measured by flow cytometry for its ability to bind anti-human Siglec-2/CD22 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human Siglec-2/CD22 Fc Chimera Alexa Fluor 488 that produces 50% of the binding response is 50.0-500 ng/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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