Recombinant Human CD22 Protein (Asp20-Arg687), C-hFc and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10270

Official Symbol

CD22

Product Overview

Biotinylated recombinant human Siglec-2/CD22 protein (Asp20-Arg687) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome.

Expression System

CHO

Species

Human

Tag

C-hFc and Avi

Predicted N Terminal

Asp20

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 104 kDa
SDS-PAGE: 120-140 kDa, under reducing conditions.

Protein length

Asp20-Arg687

Bio-activity

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is 0.1-0.9 μg/mL. Measured by its binding ability in a functional ELISA. When Human Siglec-2/CD22 (Epratuzumab) Antibody is immobilized at 0.2 μg/mL (100 μL/well), Biotinylated Recombinant Human Siglec-2/CD22 Fc Chimera Avi-tag binds with an ED50 of 1.5-18 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer, biotinylated via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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