Recombinant Human CD226 protein, C-hFc-Avi Tag, Biotinylated

Product Description

Cat

IMP-071

Official Symbol

CD226

Product Overview

Biotinylated Recombinant Human CD226 protein(AA Glu 19-Asn 247)(Accession # NP_006557) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Expression System

HEK293

Species

Human

Tag

C-hFc-Avi Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 54.4 kDa. The protein migrates as 66-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Glu 19-Asn 247

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Biotinylated Human DNAM-1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Rhesus macaque CD155, His Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human DNAM-1, Fc,Avitag with a linear range of 10-156 ng/mL.

Bioactivity-FACS 1

FACS assay shows Biotinylated Human DNAM-1, Fc,Avitag can bind to 293T cell overexpressing human CD155. The concentration of DNAM-1 is 0.03 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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