Recombinant Human CD226 protein, C-hFc-Avi Tag, Biotinylated

Product Description

Cat

IMP-071

Official Symbol

CD226

Product Overview

Biotinylated Recombinant Human CD226 protein(AA Glu 19-Asn 247)(Accession # NP_006557) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Expression System

HEK293

Species

Human

Tag

C-hFc-Avi Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 54.4 kDa. The protein migrates as 66-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Glu 19-Asn 247

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Biotinylated Human DNAM-1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Rhesus macaque CD155, His Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human DNAM-1, Fc,Avitag with a linear range of 10-156 ng/mL.

Bioactivity-FACS 1

FACS assay shows Biotinylated Human DNAM-1, Fc,Avitag can bind to 293T cell overexpressing human CD155. The concentration of DNAM-1 is 0.03 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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