Recombinant Human CD24 Protein (Ser27-Gly59), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10657
Official Symbol
CD24
Product Overview
Biotinylated recombinant human CD24 protein (Ser27-Gly59) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants.
Expression System
CHO
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Ser27
Form
Supplied as a 0.2 μm filtered solution in PBS.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 32 kDa
SDS-PAGE: 43-55 kDa, under reducing conditions
Protein length
Ser27-Gly59
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-10 Fc Chimera is coated at 5 μg/mL, the concentration of Biotinylated Recombinant Human CD24 Fc Chimera Avi-tag that produces 50% optimal binding response is found to be approximately 1.5-7.5 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-10 Fc Chimera is coated at 5 μg/mL, the concentration of Biotinylated Recombinant Human CD24 Fc Chimera Avi-tag that produces 50% optimal binding response is found to be approximately 1.5-7.5 μg/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
Phone
  • 1-631-559-9269
    1-516-512-3133
Fax
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