Recombinant Human CD244 Protein, C-hFc& AVI-tagged, Biotinylated
Product Description
Cat
IMP-1393
Official Symbol
CD244
Product Overview
Recombinant human CD244 (NP_057466.1) (Met1-Arg221) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Description
The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell–mediated cytotoxicity, induce secretion of IFN-γ and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.
Expression System
HEK293
Species
Human
Tag
C-hFc& AVI
Predicted N Terminal
Cys 22
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human CD244 consists of 453 amino acids and predicts a molecular mass of 50.80 kDa. It migrates as an approximately 65.58 kDa band in SDS-PAGE under reducing conditions.
Protein length
Met1-Arg221
Endotoxin
< 1.0 EU/μg protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Bioactivity-ELISA 1
Bioactivity-ELISA

Measured by its binding ability in a functional ELISA. Immobilized Human CD48 hFc at 2 μg/mL (100 μL/well) can bind Human 2B4/CD244 (hFc&AVI Tag), Biotinylated, the EC50 of Human 2B4/CD244 (hFc&AVI Tag), Biotinylated is 10-70 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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