Recombinant Human CD27 protein, C-Fc-His Tag
Product Description
Cat
IMP-537
Official Symbol
CD27
Product Overview
Recombinant Human CD27(NP_001233.1)(Thr21-Ile192) is expressed in HEK293, fused with a Fc, 6His tag at the C-terminus.
Description
CD27 antigen is also known as CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7, T14 and TNFRSF7. In humans, it is encoded by the CD27 gene. CD27 is a single-pass type I membrane protein with 3 TNFR-Cys repeats. It is a member of the TNF-receptor superfamily and is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. It plays a role in survival of activated T-cells and apoptosis through association with SIVA1.
Expression System
HEK293
Species
Human
Tag
C-Fc-His Tag
Form
Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl, pH 8.0.
Molecular Mass
60kDa, reducing conditions.
Protein length
Thr21-Ile192
Endotoxin
Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human TNFSF7/CD70 at 10μg/ml (100 μl/well) can bind biotinylated Human CD27/TNFRSF7, The ED50 of Human CD27/TNFRSF7 is 1.72 ug/ml.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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