Recombinant Human CD27 protein, C-Fc-His Tag
Product Description
Cat
IMP-537
Official Symbol
CD27
Product Overview
Recombinant Human CD27(NP_001233.1)(Thr21-Ile192) is expressed in HEK293, fused with a Fc, 6His tag at the C-terminus.
Description
CD27 antigen is also known as CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7, T14 and TNFRSF7. In humans, it is encoded by the CD27 gene. CD27 is a single-pass type I membrane protein with 3 TNFR-Cys repeats. It is a member of the TNF-receptor superfamily and is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. It plays a role in survival of activated T-cells and apoptosis through association with SIVA1.
Expression System
HEK293
Species
Human
Tag
C-Fc-His Tag
Form
Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl, pH 8.0.
Molecular Mass
60kDa, reducing conditions.
Protein length
Thr21-Ile192
Endotoxin
Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human TNFSF7/CD70 at 10μg/ml (100 μl/well) can bind biotinylated Human CD27/TNFRSF7, The ED50 of Human CD27/TNFRSF7 is 1.72 ug/ml.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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