Recombinant Human CD27 protein, C-Fc Tag

Product Description

Cat

IMP-259

Official Symbol

CD27

Product Overview

Recombinant Human CD27 protein(AA Thr 21-Ile 192)(Accession # NP_001233) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 45.9 kDa. The protein migrates as 53-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Thr 21-Ile 192

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human CD27, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

HPLC

The purity of Human CD27, Fc Tag was greater than 85% as determined by SEC-HPLC.

Bioactivity-ELISA 1

Immobilized Human CD27, Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human CD27 Ligand, Fc Tag with a linear range of 1-31 ng/mL.

Bioactivity-Cell based assay 1

Flow Cytometry assay shows that Human CD27, Fc Tag can bind to Raji cells Surface Human CD70. The concentration of CD27 used is 1 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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