Recombinant Human CD27 protein, C-His Tag, Biotinylated
Product Description
Cat
IMP-487
Official Symbol
CD27
Product Overview
Biotinylated Recombinant Human CD27(NP_001233.2) (Met1-Ile192) was expressed in Baculovirus-Insect Cells, fused with a C-terminal polyhistidine tag.
Description
CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.
Expression System
Insect Cells
Species
Human
Tag
C-His Tag
Predicted N Terminal
Ala 20
Form
Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass
The secreted recombinant human CD27 consists of 183 amino acids and predicts a molecular mass of 20.7 KDa.
Protein length
Met1-Ile192
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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