Recombinant Human CD27 Protein (Gln39-Pro193), N-HA-tagged
Product Description
Cat
IMP-10286
Official Symbol
CD27
Product Overview
Recombinant human CD27 Ligand/TNFSF7 protein (Gln39-Pro193) with a HA (YPYDVPDYA) tag at N-terminus was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD31-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Expression System
HEK293
Species
Human
Tag
N-HA
Predicted N Terminal
Tyr
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 23 kDa
SDS-PAGE: 24-32 kDa, reducing conditions
Protein length
Gln39-Pro193
Bio-activity
Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human CD27. The ED50 for this effect is 5-25 ng/mL in the presence of Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Noncovalently-linked homotrimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human CD27. The ED50 for this effect is 5-25 ng/mL in the presence of Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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