Recombinant Human CD27 Protein (Gln39-Pro193), N-HA-tagged
Product Description
Cat
IMP-10286
Official Symbol
CD27
Product Overview
Recombinant human CD27 Ligand/TNFSF7 protein (Gln39-Pro193) with a HA (YPYDVPDYA) tag at N-terminus was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD31-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Expression System
HEK293
Species
Human
Tag
N-HA
Predicted N Terminal
Tyr
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 23 kDa
SDS-PAGE: 24-32 kDa, reducing conditions
Protein length
Gln39-Pro193
Bio-activity
Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human CD27. The ED50 for this effect is 5-25 ng/mL in the presence of Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Noncovalently-linked homotrimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human CD27. The ED50 for this effect is 5-25 ng/mL in the presence of Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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