Recombinant Human CD27 protein, N-His Tag
Product Description
Cat
IMP-2106
Official Symbol
CD27
Product Overview
Recombinant Human CD27 protein(P26842)(Ser19~Asp188), fused with N-terminal His Tag, was expressed in E. coli.
Expression System
E. coli
Species
Human
Tag
N-His Tag
Form
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Molecular Mass
16kDa
Protein length
Ala301~Ser412
Purity
> 95%
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Western Blot 1
Western Blot

SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
The binding activity of TNFRSF7 with TNFa

TNFRSF7 (Tumor necrosis factor receptor superfamily member 7), also known as CD27 antigen, is a member of the TNF-receptor superfamily. This receptor is thought to be involved in tumor necrosis factor-activated receptor activity by binding with TNFs. Thus, a binding ELISA assay was conducted to detect the ass°Ciation of TNFRSF7 with TNFa. Briefly, recombinant human TNFRSF7 were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF7 with TNFa was shown in Figure 1 and this effect was in a dose dependent manner.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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