Recombinant Human CD274 protein, C-Fc-Avi Tag, Biotinylated
Product Description
Cat
IMP-571
Official Symbol
CD274
Product Overview
Biotinylated Recombinant Human CD274(NP_054862.1)(Phe19-Thr239) is expressed in HEK293, fused with a Fc, Avi tag at the C-terminus.
Description
CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
Expression System
HEK293
Species
Human
Tag
C-Fc-Avi Tag
Form
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Molecular Mass
70-95 kDa, reducing conditions.
Protein length
Phe19-Thr239
Endotoxin
Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Anti-Human PDL1 mAb-FC at 2μg/ml (100 μl/well) can bind Human PD-L1-Avi.The ED50 of Human PD-L1-Avi is 0.25 ug/ml.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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