Recombinant Human CD274 protein, C-Fc-Avi Tag, Biotinylated
Product Description
Cat
IMP-571
Official Symbol
CD274
Product Overview
Biotinylated Recombinant Human CD274(NP_054862.1)(Phe19-Thr239) is expressed in HEK293, fused with a Fc, Avi tag at the C-terminus.
Description
CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
Expression System
HEK293
Species
Human
Tag
C-Fc-Avi Tag
Form
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Molecular Mass
70-95 kDa, reducing conditions.
Protein length
Phe19-Thr239
Endotoxin
Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Anti-Human PDL1 mAb-FC at 2μg/ml (100 μl/well) can bind Human PD-L1-Avi.The ED50 of Human PD-L1-Avi is 0.25 ug/ml.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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